Retina and Posterior Segment
AB039. Peptidyl-prolyl cis-trans isomerase A (PPIA)—a novel biomarker of multi-episodic (recurrent) ocular toxoplasmosis
Jordan Isenberg1,2, Rubens N. Belfort1,3, Makan Golizeh4, Alexandre Da Silva5, Miguel N. Burnier1,3, Momar Ndao4
1The Henry C. Witleson Ocular Pathology Laboratory, McGill University, Montréal, Québec, Canada;2Department of Ophthalmology, University of Montreal, Montréal, Québec, Canada;3Vision Institute, Department of Ophthalmology, Federal University of São Paulo, São Paulo, SP, Brazil;4National Reference Centre for Parasitology, Research Institute of the McGill University Health Centre, Montreal General Hospital, Montréal, Québec, Canada;5Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, MD, USA
Background: Ocular toxoplasmosis (OT) is the most common etiology of posterior uveitis. The high incidence of macular scarring associated with OT is a leading cause of visual morbidity. Serum biomarkers of the disease would aid in its diagnosis. This work was designed as a pilot study to detect OT potential biomarkers.
Methods: Blood samples were collected from four groups of nine patients each; toxoplasmosis IgG- with no history of uveitis, non-toxoplasmosis uveitic, first episode OT, and symptomatic recurrent OT. Plasma serum was isolated and subjected to proteomics analysis using 2D gel electrophoresis (GE) and surface-enhanced laser desorption ionization mass spectrometry (SELDI-MS). Selected proteins were separated by GE and sequenced using tandem MS.
Results: Fifty markers of OT and 46 markers of recurrent disease were discovered by MS; 47% were cross-validated; 14 biomarkers were selected for verification by 1D-GE. 2D-GE analysis yielded 57 differentially expressed bands, 20 of which were excised and identified. One serum protein, peptidyl-prolyl cis-trans isomerase A, was validated to be a biomarker of multi-episodic OT by immunoblotting in patient and control samples.
Conclusions: This pilot study sought, for the first time, to elucidate plasma serum biomarkers for OT. This study demonstrates the potential for SELDI- MS and well as other MS technologies to identify novel disease biomarkers.
Keywords: Toxoplasmosis; uveitis; biomarker; diagnostic
doi: 10.21037/aes.2018.AB039
Cite this abstract as: Isenberg J, Belfort RN, Golizeh M, Da Silva A, Burnier MN, Ndao M. Peptidyl-prolyl cis-trans isomerase A (PPIA)—a novel biomarker of multi-episodic (recurrent) ocular toxoplasmosis. Ann Eye Sci 2018;3:AB039.
